Case #5: Improving sensitivity and resolution of analytical instruments

Problem

  1. Distribution cannot be detected by the particle size distribution analyzer, even though many sub-micron-sized particles are included in the sample.
  2. Distribution cannot be detected in sharp, even though the sample is monnodiserse.
  3. Measurement cannot be done because density of the sample is lower than the detection limit of the particle size distribution analyzer.

Solution

Contamination such as impurities and agglomeration can be removed by the centrifugation, then sensitivity of the detection by the particle size distribution analyzer can be improved.
e.g. Distribution could be detected after removing the contamination by the centrifugation.

Distribution could be detected after removing the contamination by the centrifugation

Particle concentration can be increased by the centrifugation, then distribution of the sample can be detected by the particle size distribution analyzer.
e.g. Low concentration sample was centrifuged then the particle was precipitated. Then supernatant was removed to increase the concentration.

Low concentration sample was centrifuged then the particle was precipitated

Discussion
Centrifugation can remove contamination in the sample and increase the purity of the sample. As a result, sensitivity of the detection by the particle size distribution analyzer can be increased.
Centrifugation can increase the sample concentration for better detection.

Recommended centrifuges are micro ultracentrifuge model CS150NX or CS150FNX.